Research Scientist Gilead Sciences Foster City, California
Sponsored By:
Description: This study presents an automated microdroplet technique for rapid protein therapeutic characterization using minimal sample amounts, addressing the need for faster and reliable molecular analysis in early discovery. Utilizing a combination of microdroplet generation, automated flow injection, and online MS analysis, the method proved effective for protein reduction/digestion, including complete interchain disulfide bond reduction and partial deglycosylation, with significant implications for understanding protein therapeutic candidates' structure and drug-antibody ratios (DAR) in Antibody Drug Conjugates (ADCs).
Learning Objectives:
Understand the application of microdroplet reactions in protein therapeutics.
Learn automated sample processing and analysis w.r.t microdroplet-based characterization.
Evaluate the efficacy of various microdroplet based chemical and enzymatic processes in proteins.
