Development Scientist Pion Inc. Billerica, Massachusetts
The subcutaneous injection site simulator (SCISSOR) is an in vitro analytical method that is used to probe formulation behavior without the need for excessive and costly animal testing. At the heart of the instrument lies an acellular hydrogel that mimics the subcutaneous space, responsible for imitating the chemical and architectural interactions an injectate will have after application. This talk will first aim to explain the concept of Scissor and how it works as an ‘acceptor’/’donor’ model by injecting a formulation into the simulated subcutaneous matrix (‘donor’), followed by diffusion of the formulation though a membrane to the outer ‘acceptor’ chamber, equating to uptake into the blood and lymphatic vessels in vivo. We will then describe the characterization and parameterization surrounding the artificial extracellular matrix, and how the integration of native extracellular matrix components (e.g. chondroitin sulfate, collagen, etc.) effect the release and post-injection phenomena of injectable drugs.
Learning Objectives:
Upon completion, participants will be able to understand how the events following subcutaneously injected pharmaceuticals are simulated and monitored in an in vitro environment to predict in vivo results.
Upon completion, participants will be able to describe how the dynamics of injectate-biology interactions can be probed using acellular artificial extracellular matrices.
Upon completion, participants will be able to determine the importance of in vitro testing for subcutaneous formulations and to define where the SCISSOR technology will aid in analytical characterization of pharmaceuticals.
